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. Author manuscript; available in PMC: 2023 May 18.
Published in final edited form as: Toxicol Sci. 2021 Apr 27;181(1):68–89. doi: 10.1093/toxsci/kfab009

Figure 2. Quality assessment of high-throughput transcriptomics data.

Figure 2.

(A-E) Distributions of all sample-level QC metrics, split by sample type. Dashed lines indicate thresholds for masking samples from further analysis. (F) Proportion of samples passing all QC thresholds by type. Blank = Lysis buffer negative controls containing no cellular material; QC Sample = samples prepared in larger batches and added to each plate prior to conduct of TempO-Seq assay; DMSO Control = vehicle control for all other wells; Ref Chem = single dose reference chemical treatments; Test Sample = wells treated with a test chemical.