TABLE 2.
Strains used in this study
| Strain, phage, or plasmid | Genotype | Source or referencea |
|---|---|---|
| E. coli | ||
| ECL116 | F− ΔlacU169 endA hsdR thi | 1 |
| LS854 | trpA9605 his-85 rpsL136 Δcrp-3 metE70 trpR55 λ− IN(rrnD-rrnE)1 | CGSC |
| JW375 | supE42 λ− zhc-511::Tn10 | CGSC |
| L8854 | trpA9605 his-85 rpsL136 Δcrp-3 zhc-511::Tn10 metE70 trpR55 λ− IN(rrnD-rrnE)1 | E. Elsinghorst |
| SME1035 | ECL116 λSME103 recA::cat | 10 |
| SME1036 | ECL116 λSME104 recA::cat | 10 |
| SME1037 | ECL116 λSME105 recA::cat | 10 |
| SME1088 | ECL116 λSME104 ΔrhaS recA::cat | 10 |
| SME1089 | ECL116 λSME105 ΔrhaS recA::cat | 10 |
| SME1222 | ECL116 λSME103 ΔrhaS | 2 |
| SME1853 | ECL116 λSME101 Δcrp-3 zhc-511::Tn10 | This study |
| SME1854 | ECL116 λSME103 Δcrp-3 zhc-511::Tn10 | This study |
| SME1855 | ECL116 λSME104 Δcrp-3 zhc-511::Tn10 | This study |
| Phages | ||
| λSME101 | λ RS45 Φ(rhaB-lacZ)Δ226 | 10 |
| λSME103 | λ RS45 Φ(rhaB-lacZ)Δ110 | 10 |
| λSME104 | λ RS45Φ(rhaB-lacZ)Δ84 | 10 |
| λSME105 | λ RS45Φ(rhaB-lacZ)Δ70 | 10 |
| Plasmids | ||
| pREIIα | Ampr pREIIα rpoA | 3 |
| pHTf1α | Ampr pHTf1α rpoA | 31 |
| pSE186 | Ampr pHG165crp+ | This study |
| pSE187 | pSE186 (Thr158Ala) | This study |
| pSE188 | pSE186 (Gly162Ala) | This study |
| pSE189 | pSE186 (His19Ala) | This study |
| pSE190 | pSE186 (His21Ala) | This study |
| pSE191 | pSE186 (Lys101Ala) | This study |
| pSE192 | Ampr pREIIα rpoA (α-Δ235) | This study |
a
CGSC, E. coli Genetic Stock Center, Yale University, New Haven, Conn.