TABLE 5.
Effect of expressing an α-CTD deletion derivative at rhaBAD
| Promoter fusion | β-Gal sp acta
|
|||
|---|---|---|---|---|
|
rhaS+
|
ΔrhaS
|
|||
| wt α | α-Δ235 | wt α | α-Δ235 | |
| Φ(rhaB-lacZ)Δ110 | 330 | 26 | 0.018 | <0.01 |
| Φ(rhaB-lacZ)Δ84 | 8.7 | 0.16 | <0.01 | <0.01 |
| Φ(rhaB-lacZ)Δ70 | 0.056 | <0.01 | <0.01 | 0.010 |
a
β-Galactosidase (β-Gal) specific activity was measured from single copy rhaB-lacZ fusions in rhaS+ or ΔrhaS strains expressing either wild-type (wt) α or an α-CTD deletion derivative, α-Δ235. Cultures were grown in MOPS growth media containing glycerol, l-rhamnose, and 125 μg of ampicillin per ml. Standard errors were less than 44% of the average units.