Table 3.
Stimulation of SCE by HO-induced DSBs in rad53 and chk1 mutant strains
| Genotype (strain)a | % Viability after HO inductionb |
His+recombinants/Trp+CFU × 105 |
Fold increasee | Trp+CFU/total CFU (%) |
||
|---|---|---|---|---|---|---|
| Before HO inductionc |
After HO inductiond |
Before HO induction |
After HO induction |
|||
| RAD (YB163) | 84 ± 18 | 5.8 ± 1 | 76 ± 13 | 13 | 94 ± 3 | 91 ± 6 |
| sml1 (YB227) | 77 ± 22 | 7.9 ± 0.3 | 101 ± 33 | 13 | 93 ± 2 | 92 ± 1 |
| rad53 sml1 (YB231) | 69 ± 16 | 28 ± 14 | 93 ± 43 | 3.3 | 89 ± 6 | 87 ± 7 |
| rad53(YB230) | 91 ± 9 | 5.3 ± 0.6 | 17 ± 3 | 3.2 | 90 ± 1 | 91 ± 3 |
| rad53 pds1 (YB358) | 65 ± 8 | 4.7 ± 0.8 | 26 ± 9 | 5.5 | 84 ± 6 | 79 ± 4 |
| chk1 (YB335) | 56 ± 5 | 12 ± 9 | 161 ± 74 | 13 | 96 ± 2 | 95 ± 1 |
a
For complete genotype, see Table 1.
b
Trp+ CFU after HO induction/Trp+ CFU before HO induction × 100%.
c
His+ recombinants before HO induction/Trp+ CFU before HO induction.
d
His+ recombinants after HO induction/Trp+ CFU after HO induction.
e
His+ frequency after HO induction/His+ frequency before HO induction.