Table 5.
Stimulation of SCE by MMS and 4-NQO in wild-type, sml1, rad53 and chk1 strains
| Genotype (strain)a | His+/CFU × 106 (avg) after 4-NQO exposure (survival %) |
His+/CFU × 106 (avg) after MMS exposure(survival%) |
||||||
|---|---|---|---|---|---|---|---|---|
| Controlb | 2 μM | 10 μM | Ratioc | Controlb | 2 mM | 10 mM | Ratioc | |
| RAD (YB163) | 15 ± 4 | 74 ± 20 (71) | 164 ± 48 (30) | 5, 11 | 15 ± 5 | 59 ± 12 (92) | 118 ± 27 (60) | 4, 8 |
| sml1 (YB227) | 10 ± 1 | 96 ± 20 (69) | 323 ± 18 (9) | 10, 32 | 10 ± 1 | 51 ± 1 (100) | 99 ± 5 (70) | 5, 10 |
| rad53 sml1 (YB229) | 23 ± 2 | 53 ± 16 (51) | 79 ± 30 (2) | 2, 3 | 21 ± 2 | 54 ± 4 (75) | 42 ± 5 (24) | 3, 2 |
| rad53 (YB230) | 16 ± 7 | 28 ± 14 (61) | 73 ± 20 (7) | 2, 4 | 24 ± 10 | 37 ± 7 (70) | 81 ± 15 (8) | 2, 3 |
| chk1(YB335) | 19 ± 1 | 130 ± 42 (52) | 400 ± 190 (20) | 7, 21 | 19 ± 1 | 62 ± 17 (71) | 163 ± 15 (71) | 3, 8 |
a
For complete phenotype, see Table 1.
b
Frequency is events/viable cells; n = 3.
c
His+ frequency w/agents/spontaneous His+ frequency; first and second numbers indicate ratios for lower and higher concentrations, respectively.