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. 2022 Dec 6;22(3):263–273. doi: 10.1093/bfgp/elac044

Figure 5.

Figure 5

BCR-genes influence pseudo-temporal ordering of bone marrow early B-lineage cells. A, Cartoon depicting the developmental stages of BM early B-lineage cells, with the expression of CD34, MKI67 and CXCR5 indicated. B-D, UMAP projection of unsupervised clustering without BCR-genes. E-G, UMAP projections of unsupervised clustering with BCR-genes retained. UMAP projection coloured according to scaled gene expression of CD34  (B and E), MKI67 and CXCR5  (C and F). D and G, UMAP projection coloured according to maturation level inferred from the pseudo-temporal analysis, with super-imposed developmental trajectory inferred from Monocle 3. Arrow shows the developmental origin (CD34-expressing cells). H, UMAP projection of unsupervised clustering with BCR-genes retained, with clusters 6, 7, and 10 highlighted with ellipses. I, UMAP projection of unsupervised clustering without BCR-genes, with the cells from clusters 6, 7, and 10 in Figure 5H highlighted.