(a) Schematic of doxycycline intervention in wildtype (WT) and TAZKD littermates. (b) Body mass of WT and TAZKD mice (n =6 for WT, n =5 for TAZKD). (c) Body composition of WT and TAZKD mice (n =6 for WT, n =5 for TAZKD). (d) TAZ mRNA abundance in skeletal muscle from WT and TAZKD mice (n =4 for WT, n =6 for TAZKD). (e) Abundance of individual CL species in isolated mitochondria (n =5 for both groups). TLCL is shown in red asterisk. (f) TLCL to total CL ratio (n =5 for both groups). (g) Rates for O2 consumption, ATP production, and P/O ratio in skeletal muscles from WT and TAZKD mice with 200 μmol/L of ADP (n =6 for WT, n =8 for TAZKD). (h) Proposed mechanism for how weight loss improves OXPHOS efficiency. Two-way ANOVA with Sidak multiple comparisons (c, e) or unpaired t-test (b, d, f, g). All P-values indicate statistical significance between WT and TAZKD groups. Data are represented as mean ± SEM.