Extended Data Table 1. Analysis of platelet count, size, and surface expression of glycoproteins in Gp5dThr mice.
Mean platelet count and size were determined using a Sysmex cell counter. Surface expression of platelet glycoproteins was determined by flow cytometry. Diluted whole blood was stained with FITC-labelled antibodies at saturating amounts for 15 min at RT. Platelets were analysed immediately on a FACSCalibur. Results are expressed as mean fluorescence intensity (MFI) ± SD. n=4–6, two-tailed unpaired t-test with Welch’s correction, *p < 0.05.
| WT | Gp5dThr | P-value | Sig. | |
|---|---|---|---|---|
|
| ||||
| Count | 85.8 ±25.0 | 102.8 ±20.4 | 0.2460 | n.s. |
| MPV | 5.4 ±0.2 | 5.5 ± 0.2 | 0.4678 | n.s. |
| WBC [10/μl] | 76.7 ±21.6 | 76.0 ±15.2 | 0.95355473 | n.s. |
| RBC [1E4/μl] | 89.0 ± 25 | 93.2 ±3,1 | 0,70015665 | n.s. |
| HGB [g/μl] | 1.4 ±0.3 | 1.5 ± 0.1 | 0.43026063 | n.s. |
| HCT [%] | 4.8 ± 1.3 | 5.1 ±0.2 | 0.52205469 | n.s. |
| GPIb [MFI] | 341.3 ±20.3 | 354.3 ± 5.3 | 0.29312226 | n.s. |
| GPV [MFI] | 318.5 ± 11.9 | 289 ±32.6 | 0.1203 | n.s. |
| GPIX [MFI] | 308.75 ± 8.66 | 317.75 ±4.79 | 0.13244492 | n.s. |
| GPVI [MFI] | 33.25 ±0.96 | 34 ±1.41 | 0.41799623 | n.s. |
| αIIbβ3 [MFI] | 338.75 ± 27.68 | 353 ± 14.53 | 0.4216117 | n.s. |
| β3 [MFI] | 157.8 ±43.6 | 153.2 ±5.7 | 0.8260 | n.s. |
| α5 [MFI] | 18 ±0.01 | 18 ±0.03 2 | 1 | n.s. |
| α2 [MFI] | 38.75 ± 0.957 | 39.25 ±3.59 | 0.80343776 | n.s. |
| CD9 [MFI] | 669.75 ±45.15 | 746 + 2.83 | 0.04258772 | * |
| CLEC-2 [MFI] | 90.75 ± 6.24 | 82.5 ± 15.26 | 0.37396966 | n.s. |
| CD84 [MFI] | 25 ± 1.63 | 25.5 ±0.58 | 0.59667799 | n.s. |