Figure 1 .

GRh3 significantly inhibits the proliferation of CRC cells in vivo and in vitro

(A) The chemical structure of GRh3. (B‒G) The cell viability of CRC cells (HT29, HCT116, RKO, SW620, and DLD1 cells in that order) and HCoEpiC cells treated with different concentrations of GRh3 in medium for 24 and 48 h ( n=3). (H,I) Giemsa-stained colonies were observed under an inverted microscope and quantified ( n=3). HT29 and HCT116 human CRC xenograft mouse models were treated with solvent or GRh3 (20 mg/kg/d). (J) Body weight was measured every 7 days ( n=10). (K) HE staining of liver and kidney tissues. Scale bar= 100 μm. (L) Tumor size was measured every 7 days ( n=10). (M) Representative images of HT29 and HCT116 xenograft tumor tissues from the control (solvent) and GRh3-treated groups. (N) Xenograft tumor tissue weight after 21 days of treatment ( n=10). Data are presented as the mean±SD of triplicate experiments. (B‒G) * P<0.05, *** P<0.001 compared with the blank group (0 μM GRh3); ## P<0.01, ### P<0.001 compared with the 24 h control group; (I,J,L,N) *** P<0.001 compared with the blank/control group.