Figure 5 .

Pyroptosis and ferroptosis in CRC cells activated by GRh3 are regulated by the Stat3/p53/NRF2 axis

(A) Viability of HT29 cells and HCT116 cells pretreated with or without PFT-α (p53 inhibitor) followed by treatment with GRh3. (B) IL-1β released into the culture medium was detected by ELISA. (C) IL-18 released into the culture medium was detected by ELISA. (D) Relative concentrations of lipid ROS in HT29 and HCT116 cells. (E) Relative concentrations of GSH in HT29 and HCT116 cells. (F) Iron concentrations in HT29 and HCT116 cells. (G) Concentrations of MDA in HT29 and HCT116 cells. (H) Representative western blots of the Stat3/p53/NRF2 axis, pyroptosis, and ferroptosis-related protein expression in HT29 and HCT116 cells exposed to GRh3-containing medium for 48 h with or without PFT-α pretreatment for 4 h. (I,J) Relative expression levels of Stat3/p53/NRF2 axis-, pyroptosis-, and ferroptosis-related proteins in HT29 and HCT116 cells. Data are presented as the mean±SD. * P<0.05, ** P<0.01, *** P<0.001, vs the blank group (0 μM GRh3). # P<0.01, ## P<0.01, ### P<0.001 vs the 40 μM GRh3 group; n=3.