Figure 8 .

SRCIN1 reverses the regulatory effect of miR-150-3p on SW620 cells

SW620 cells were transfected with NC mimic+Vector, NC mimic+SRCIN1 overexpression vector, miR-150-3p mimic+Vector, or miR-150-3p mimic+SRCIN1 overexpression vector. (A) Viability of SW620 cells at 24, 48, and 72 h. (B) Protein levels of cyclin D1, CDK6, Bcl-2, Bax and cleaved caspase 3 in SW620 cells with the indicated transfection were determined by western blot analysis. GAPDH was used as a loading control. (C) EdU assay was used to detect SW620 cell proliferation. (D) Would healing assay was used to detect SW620 cell migration. (E,F) Transwell assay was used to detect SW620 cell migration and invasion. Data are presented as the mean±standard deviation. ** P<0.01, *** P<0.001.