Evaluation of emergency administration of t-TLNPs in restoring hemostatic efficacy in liver laceration bleeding model in anticoagulated mice. (A) Schematic and representative anatomic picture of liver laceration model in mice where treatment (sham saline, UNP, or t-TLNP) was administered post-injury and blood loss from the injured liver was measured by preweighed gauze. (B) Blood loss data (in grams, g) from mouse liver injury model studies showing a significant increase in bleeding from an injured liver in defect (heparinized) mice in comparison to normal (nonheparinized) mice. Treatment with UNPs was unable to reduce blood loss, but treatment with t-TLNPs was able to significantly reduce blood loss. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.