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. 2023 Jan 28;101:skad035. doi: 10.1093/jas/skad035

Table 11.

Relative abundance of jejunal mucosa-associated microbiota at family level of nursery pigs fed diets with varying levels of zinc glycinate (ZnGly) and zinc oxide (ZnO) under F18+E. coli challenge1

Item NC2 PC ZnGly at 400 mg/kg ZnGly
800 mg/kg		 ZnGly
1,200 mg/kg		 ZnO 2,500 mg/kg P-value
ZnO
0 mg/kg		 ZnO
700 mg/kg		 ZnO
1,400 mg/kg		 ZnO
2,357 mg/kg		 SEM NC vs. PC3 PC vs. ZnO 2,500 mg/kg ZnO
Linear4 		ZnO
Quad.4 		ZnGly
Linear5 		ZnGly Quad.5 Zn
Source6 		PC vs. Zn source7
Jejunal mucosa
 Alcaligenaceae 2.19 1.83 2.07 2.10 1.97 2.49 2.40 2.97 2.04 0.29 0.241 0.475 0.201 0.258 0.001 0.441 0.933 0.383
 Bacillaceae 2.42 2.36 2.12 2.63 2.22 2.05 2.17 2.28 2.57 0.25 0.834 0.506 0.502 0.181 0.841 0.438 0.159 0.958
 Burkholderiaceae 1.56 1.57 1.27 1.41 1.47 1.36 1.45 1.38 1.27 0.17 0.964 0.210 0.702 0.445 0.612 0.497 0.995 0.150
 Clostridiaceae 16.10 16.22 13.84 17.34 13.56 12.69 12.15 13.47 16.48 1.31 0.951 0.888 0.203 0.170 0.097 0.165 0.161 0.514
 Comamonadaceae 2.33 3.14 4.85 3.19 3.60 4.21 3.39 3.76 3.51 0.72 0.382 0.684 0.685 0.090 0.891 0.304 0.150 0.193
 Deinococcaceae 2.06 1.86 1.59 1.91 1.62 1.73 1.72 1.68 1.83 0.19 0.423 0.891 0.855 0.636 0.606 0.503 0.334 0.474
 Enterobacteriaceae 6.45 7.02 5.19 4.85 6.36 5.17 5.19 6.80 6.10 0.64 0.482 0.254 0.648 0.334 0.796 0.004 0.257 0.051
 Flavobacteriaceae 0.97 1.14 1.01 1.14 1.06 1.09 1.61 1.23 1.16 0.16 0.432 0.920 0.823 0.790 0.221 0.417 0.488 0.776
 Lactobacillaceae 0.47 1.33 0.85 0.42 1.13 0.29 0.52 1.14 0.71 0.50 0.228 0.385 0.619 0.626 0.684 0.276 0.845 0.373
 Methylococcaceae 0.67 0.77 1.65 0.94 1.07 1.22 1.33 0.57 1.20 0.37 0.820 0.363 0.476 0.200 0.528 0.016 0.331 0.110
 Micromonosporaceae 1.42 1.36 1.18 1.77 1.80 1.49 2.03 1.99 1.66 0.29 0.841 0.441 0.530 0.126 0.037 0.846 0.251 0.822
 Mycobacteriaceae 2.26 2.02 1.75 2.01 1.88 1.52 1.86 2.05 1.83 0.20 0.417 0.500 0.328 0.161 0.822 0.257 0.795 0.355
 Nocardiaceae 0.86 1.35 1.37 1.20 1.11 1.37 1.38 2.05 1.29 0.24 0.132 0.834 0.991 0.339 0.047 0.158 0.797 0.926
 Pseudoalteromonadaceae 2.87 3.40 2.79 3.20 3.16 2.68 3.00 2.69 3.25 0.23 0.118 0.666 0.609 0.067 0.071 0.535 0.166 0.195
 Pseudomonadaceae 3.45 3.38 3.76 3.53 3.35 4.17 3.43 3.53 3.41 0.28 0.861 0.955 0.292 0.048 0.931 0.590 0.326 0.527
 Rhizobiaceae 3.71 2.51 2.71 4.63 2.84 2.37 2.96 2.44 1.83 0.91 0.334 0.583 0.439 0.239 0.993 0.679 0.478 0.823
 Sphingomonadaceae 5.59 6.77 9.26 5.46 8.54 7.54 8.91 6.80 4.90 0.92 0.362 0.152 0.634 0.198 0.946 0.015 0.001 0.787
 Staphylococcaceae 13.08 12.12 11.51 11.18 11.96 11.24 10.57 11.57 12.29 1.16 0.529 0.911 0.972 0.820 0.594 0.461 0.612 0.870
 Streptomycetaceae 6.25 5.52 6.56 6.00 6.69 9.41 7.18 7.21 5.60 0.66 0.437 0.935 0.002 0.028 0.059 0.447 0.306 0.492
 Xanthomonadaceae 1.79 2.30 1.83 1.79 2.01 2.57 4.38 1.98 2.00 0.64 0.562 0.731 0.359 0.674 0.565 0.124 0.849 0.613

1Data represent the least square means of 8eight replications except for three treatments: (1) one pig in NC group, (2) one pig in the group fed ZnO at 2,500 mg/kg, and (3) one pig in the group fed ZnGly at 400 mg/kg and ZnO at 700 mg/kg. Pigs had to be removed due to severe BW loss following the IACUC guideline after the statistical confirmation as outliers.

2No challenge.

3F18+E. coli challenge on d 7 after weaning.

4Dose–response effects of ZnO supplementation (ZnO at 0, 700, 1,400, and 2,357 mg/kg) with ZnGly at 400 mg/kg.

5Dose–response effects of ZnGly supplementation [ZnGly at 0 (PC), 400, 800, and 1,200 mg/kg].

6ZnGly at 400 mg/kg vs. ZnO at 2,500 mg/kg.

7PC vs. ZnGly at 400 mg/kg + ZnO at 2,500 mg/kg.