Table 9.

Alpha-diversity in jejunal mucosa-associated microbiota of nursery pigs fed diets with varying levels of zinc glycinate (ZnGly) and zinc oxide (ZnO) under F18⁺E. coli challenge¹

Item	NC2	PC	ZnGly at 400 mg/kg				ZnGly 800 mg/kg	ZnGly 1,200 mg/kg	ZnO 2,500 mg/kg		P-value
			ZnO 0 mg/kg	ZnO 700 mg/kg	ZnO 1,400 mg/kg	ZnO 2,357 mg/kg				SEM	NC vs. PC3	PC vs. ZnO 2,500 mg/kg	ZnO Linear4	ZnO Quad.4	ZnGly Linear5	ZnGly Quad.5	Zn Source6	PC vs. Zn source7
Jejunal mucosa
Chao1	146.7	150.6	148.7	146.4	147.8	142.4	149.4	144.6	145.7	2.4	0.182	0.096	0.048	0.473	0.065	0.472	0.301	0.181
Shannon	3.96	4.02	3.99	3.99	4.02	4.08	4.06	4.03	4.02	0.04	0.211	0.956	0.039	0.503	0.480	0.966	0.470	0.724
Simpson	0.947	0.952	0.955	0.951	0.958	0.962	0.961	0.958	0.953	0.003	0.328	0.869	0.070	0.364	0.108	0.374	0.629	0.639

¹Data represent the least square means of eight replications except for three treatments: 1) one pig in NC group, 2) one pig in the group fed ZnO at 2,500 mg/kg, and 3) one pig in the group fed ZnGly at 400 mg/kg and ZnO at 700 mg/kg. Pigs had to be removed due to severe BW loss following the IACUC guideline after the statistical confirmation as outliers.

²No challenge.

³F18⁺E. coli challenge on d 7 after weaning.

⁴Dose response effects of ZnO supplementation (ZnO at 0, 700, 1,400, and 2,357 mg/kg) with ZnGly at 400 mg/kg.

⁵Dose response effects of ZnGly supplementation [ZnGly at 0 (PC), 400, 800, and 1,200 mg/kg].

⁶ZnGly at 400 mg/kg vs. ZnO at 2,500 mg/kg.

⁷PC vs. ZnGly at 400 mg/kg + ZnO at 2,500 mg/kg.