FIG. 6.

Northern analyses of minvac and minvac double transformants to detect gvpA and gvpF-M mRNA. RNA samples were derived from the exponential (e) and stationary (s) growth phases. A 10-μg sample of total RNA was applied to each slot. Transcripts starting at the pA promoter were visualized using the A probe (A), and transcripts derived from the pF promoter were visualized using an FG probe (FG), whereas the LM probe also detected transcripts starting at the fdx promoter in pJSA35 in the cases of the I-M and J-M constructs (LM). The values on the right side of each gel are the sizes (in kilobases) of the hybridizing mRNAs.