Extended Data Fig. 3. Mitochondrial translation and OXPHOS complex integrity in the MitoKO DdCBE-treated cells.
a. Densitometric quantification of the mitochondrial de novo protein synthesis gels presented in Fig. 4c. WT (dark grey line) was used as baseline control in each mtDNA protein-encoded gene. The top panel refers to the 16% Tris-Gly gel and the bottom panel refers to the 16% Tricine gel. Source data are provided as a Source Data file. b. Immunoblotting of a BNGE with antibodies specific to each mitochondrial complex: NDUFB8 (CI), UQRC2 (CIII) COX IV (CIV) and ATP5A (CV), in mitochondria isolated from NIH/3T3 cells that underwent four rounds of MitoKO DdCBE treatment with the Nd4l, Nd5, Nd6, CoIII, Atp8 pairs and WT controls. An antibody specific for complex II (SDHB) was used as loading control. Uncropped scans are provided as a Source Data file.