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. 2023 May 18;6:535. doi: 10.1038/s42003-023-04916-w

Table 1.

Cryo-EM data collection, refinement, and validation statistics.

HIV-1AD8 Env
(EMD-28953)
(8FAD)
HIV-1AE2 Env
(EMD-28954)
(8FAE)
Data collection and processing
Magnification 105,000 105,000
Voltage (kV) 300 300
Electron exposure (e/Å) 54 52/56/62
Defocus range (μm) −1.0 to −2.7 −1.0 to −3.0
Pixel size (Å) 0.685 0.825
Symmetry imposed C1 C1
Initial particle images (no.) 365,824 1,209,493
Final particle images (no.) 37,797 140,035
Map resolution (Å) 4.1 3.8
FSC threshold 0.143 0.143
Map resolution range (Å) 3.6–8 3.6–8
Refinement
Initial model used (PDB code) 7N6U 7N6U
Model resolution (Å) 4.0 3.9
  FSC threshold 0.143 0.143
Model resolution range (Å) 3.5–8 3.5–8
Map sharpening B factor (Å2) −80 −80
Model composition
 Non-hydrogen atoms 16,210 17,693
 Protein residues 1797 1832
 Ligands 145 250
B factors (Å2)
   Protein 86.11 184.36
   Ligands 133.64 105.59
R.m.s. deviations
Bond lengths (Å) 0.010 0.012
Bond angles (degree) 1.471 1.596
Validation
MolProbity score 2.51 2.55
Clashscore 10.10 8.21
Poor rotamers (%) 3.58 5.71
Ramachandran plot
Favored (%) 89.48 90.57
Allowed (%) 9.57 8.49
Disallowed (%) 0.96 0.94