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. 2023 May 19;9:171. doi: 10.1038/s41420-023-01466-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A, B KYSE-30 and KYSE-150 cells were treated by rMV-Hu191 at an MOI of 0.1 for 96 h, and then the cell morphology was analyzed by an optical microscope. The scale bars represent 50 μm. C, D KYSE-30 and KYSE-150 cells were treated by rMV-Hu191 at the indicated MOI for 96 h. Cell lysates were analyzed by immunoblot to detect the cleavage of caspase-3 and GSDME. GSDME-FL full-length GSDME, GSDME-N the N-terminal cleavage product of GSDME. MV-N MV-nucleoprotein. E, F KYSE-30 and KYSE-150 cells were treated by rMV-Hu191 at an MOI of 0.1 for the indicated time. Cell lysates were analyzed by immunoblot to detect the cleavage of caspase-3 and GSDME. G, H rMV-Hu191 induced LDH release in KYSE-30 and KYSE-150 cells. Cells were treated by rMV-Hu191 at the indicated doses and time. I, J KYSE-30 and KYSE-150 cells treated by rMV-Hu191 at an MOI of 0.1 for 48 h were collected and stained with Annexin V-FITC/PI and then subjected to flow cytometry analysis.