TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild type | 10 |
| PAO-MW20 | rpoS mutant of PAO1, Gmr | This study |
| PAO-R1 | ΔlasR derivative of PAO1, Tcr | 9 |
| PAO-MW1 | ΔlasI ΔrhlI derivative of PAO1, Hgr Tcr | 31 |
| PDO111 | rhlR::Tn501 derivative of PAO1, Hgr | 3 |
| E. coli | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17 deoR gyrA96 thi-1 relA1 supE44 | 26 |
| S17-1 | thi pro hsdR recA RP4-2 (Tet::Mu) (Km::Tn7) | 28 |
| Plasmids | ||
| pCR2.1 | TA cloning vector, Apr | Invitrogen |
| pEX1.8 | Broad-host-range expression vector, Apr | 23 |
| pSUP102 | pACYC184 carrying mobRP4, Cmr Tcr | 27 |
| pRK2013 | tra+ helper plasmid for triparental matings, Kmr | 5 |
| pHRP315 | Ω Smr/Spr cassette, Apr | 20 |
| pHRP309 | Promoterless lacZ, Gmr | 20 |
| pTL61T | lacZ transcriptional fusion vector, Apr | 17 |
| pBBR1MCS-5 | Broad-host-range vector, Gmr | 13 |
| pRPOS-1 | pCR2.1 containing a 2.8-kb rpoS fragment | This study |
| pRPOS-Gm | pRPOS-1 with aacC1 interrupting the rpoS gene, Gmr | This study |
| pMW102 | rpoS::aacC1 on pSUP102, mobilizable rpoS knockout plasmid, Gmr | This study |
| pMW105 | pEX1.8 containing ptac-rpoS, Apr | This study |
| pMW301 | Broad-host-range hcnAB-lacZ reporter, Apr Cbr | This study |
| pMW303 | Broad-host-range phzABC-lacZ reporter, Apr Cbr | This study |
| pMW304 | Broad-host-range rhlR-lacZ reporter, Gmr Spr | This study |
| pMW305 | Broad-host-range rhlI-lacZ reporter, Gmr Spr | This study |
| pTn5-Gm | pUTmini-Tn5 containing aacC1, Gmr | This study |
| pJBA24 | Cloning vector for creating reporter fusions on Tn5, Apr | 1 |
| pMRP24-RPOS | pJBA24 containing rpoS promoter, Apr | This study |
| pMW24-RPOS | rpoS promoter-lacZ fusion vector, Apr | This study |
| pUTminiTn5-Gm | Delivery plasmid for mini-Tn5-Gm, Apr Gmr | This study |
| pTn5-RPOS | pUTmini-Tn5 carrying rpoS-lacZ from pMW24-RpoS | This study |