FIG. 3.

Growth of E. coli EC1250 and deletion mutants. Cells were grown in a microtiter plate as described in Materials and Methods (150-μl cultures). The optical density at 600 nm (OD_{600 nm}) was recorded with a microtiter plate reader every 5 min over 24 h. Every fifth measurement is shown. Only the growth profiles obtained with mutants grown on 1,3-dioxo-2-isoindolineethanesulfonate (A), butanesulfonate (B), PIPES (C), and MOPS (D) are shown. The optical densities obtained ranged from 0.25 to 0.5 and corresponded to optical densities of 1.0 to 2.0 when measured with a 1-cm light path in a Uvikon P-810 spectrophotometer.