Figure 1.
Cerebrovascular dilation. Middle cerebral artery (MCA) (a) dose-response curve for endothelial-dependent dilation (EDD) to acetylcholine (ACh), (b) maximal response of the MCA to the acetylcholine (ACh; 10−4 M) with and without the acute (30 min) incubation of L-NAME, the µm change values reflect the nitric oxide (NO)-dependent dilation = (Max DilationACh) – (Max DilationACh+L-NAME), (c) MCA dose-response curve for endothelial-independent dilation (EID) to sodium nitroprusside (SNP), and (e) the vasoconstrictor response to phenylephrine (PE) in control and UCMS mice with (+Feb) or without chronic febuxostat treatment. *p < 0.05 vs control; ^p < 0.05 vs control + Feb; +p < 0.05 vs UCMS + Feb; #p < 0.05 vs no incubation experiment within the same group. mean ± SD. n = 16–20 mouse MCA’s/group. Repeated measured ANOVA with a group-by-time interaction was performed and when a significant interaction (panel A and B only) was established a tukey post-hoc test was explored.