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(A) The YbeN-catalyzed adenylation reaction. (B and C) HPLC analyses of the YbeN-catalyzed adenylation reactions: (B) 1.5 mM NAMN, reaction time is 15 min; (C) 1.5 mM NMN, reaction time is 40 min. (D and E) HPLC analysis of the competitive adenylation of NAMN and NMN: (D) the reaction mixture at t = 5 min; (E) the reaction mixture at t = 0 min. Labeled peaks: 1, NAMN; 2, NMN; 3, ATP; 4, NAAD; 5, NAD.
