Time to peak metabolism (h) of the three P. aeruginosa biofilms - PAO1 (A), PA14 (B), and PA10548 (C) against tobramycin [1], gentamicin [2], ciprofloxacin [3], and chlorhexidine [4]. Biofilms were either untreated or CAP treated for 90 s followed by the addition of antimicrobial. Error bars indicate the mean ± standard deviation determined from biological replicates, asterisks indicate significant differences between the untreated and CAP-treated groups (p < 0.05, p < 0.01, p < 0.001, and p < 0.0001) using one-way ANOVA and Dunnett's post-test analysis (n = 3).