Figure 5.

The therapeutic performances of PdH injection plus hydrogen inhalation for alleviating moderate NASH. Model building and therapeutic procedures (A), the levels of serum cytokines including IL-1β (B), IL-6 (C), TNF-α (D), IHC and immunofluorescence detection of α-SMA, CD68 and F4/80 levels in liver sections (E), and the H&E, Oil Red O (black arrow towards red spots representing neutral triglycerides and lipids) and Masson's trichrome staining (blue representing collagen) images of liver sections at the end of treatment (F). All the data were presented as mean ± standard deviation (Mean ± SD) with individual data. Student t-test was applied for comparison between groups. Difference was considered significant using asterisk as follows: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.