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. 2023 May 5;20:100662. doi: 10.1016/j.mtbio.2023.100662

Fig. 4.

Fig. 4

Differences in cell characteristics of IMRCs cultured on monolayers and different microcarriers in stirred bioreactors. A. Live/dead and phalloidin/DAPI staining of IMRCs on different microcarriers at 3 ​h (Green: live cells, cytoskeleton; red: dead cells; blue: cell nucleus). Scale bar, 50 ​μm. B. Live/dead staining of IMRCs on different microcarriers after 2 days, 4 days, and 6 days. (Green: live cells, red: dead cells). Scale bar, 50 ​μm. C. Attachment efficiency of IMRCs cultured for 3 ​h in bioreactors. D. Growth curves of IMRCs cultured on different microcarriers in bioreactors. E. Cell density of IMRCs cultured on different microcarriers after 6 days in bioreactors. F. Multipotency of IMRCs harvested from monolayers or microcarriers was examined by differentiation into osteogenic, adipogenic and chondrogenic lineages. Scale bar, 100 ​μm. G. Cell proliferation of IMRCs harvested from monolayers or microcarriers and cultured in cell culture plates. H. Cell adhesion and morphology of IMRCs harvested from monolayers or microcarriers and inoculated into cell culture plates. Scale bar, 100 ​μm. I. Cell senescence detection by β-galactosidase (β-gal) staining of monolayers or different microcarriers. Scale bar, 100 ​μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)