Fig. 5.

Transcriptome profiles of IMRCs harvested from monolayers or microcarriers in stirred bioreactors. A. Principal component analysis (PCA) of RNA-Seq of the monolayer group and microcarrier groups. B. Volcano map of differentially expressed genes (DEGs) of IMRCs harvested from microcarriers vs. monolayer. Blue dots indicate significantly downregulated DEGs; red dots indicate significantly upregulated DEGs; grey dots indicate no significant difference. C. Heatmap of DEGs in IMRCs among the monolayer group and different microcarrier groups. D. GO terms of microcarrier groups. BP, biological processes; CC, cellular component; MF, molecular function. E. KEGG pathway enrichment of microcarrier groups. The black number is the number of enriched genes. F. GO terms of the Cytodex-3 group vs. HAMC group. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)