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. 2023 Apr 12;299(5):104699. doi: 10.1016/j.jbc.2023.104699

Figure 1.

Figure 1

The phosphorylation of EphA2 at Ser-897 is induced by cellular stress via p38.A, HeLa cells were stimulated with 10 ng/ml EGF for 10 min or 50 μM anisomycin (Aniso) for 20 min. B and C, HeLa cells were treated with 10 μM SB203580 (SB), BI-D1870 (BI), BIRB796 (BIRB), Skepinone-L (Sk-L), or VX-702 (VX) for 30 min and then stimulated with 10 ng/ml EGF for 10 min or 50 μM anisomycin for 20 min. D, HeLa cells were transfected with siRNAs against p38α or the negative control. At 72 h post-transfection, cells were stimulated with 50 μM anisomycin for 20 min. E, HeLa cells were treated with 10 μM SB203580 and then stimulated with 100 μM cisplatin (CDDP) for 3 h or 0.3 M NaCl (Osmo) for 10 min. whole-cell lysates were immunoblotted with primary antibodies against phospho-EphA2 at Ser-897 (pS-EphA2), EphA2, phospho-ERK (pERK), ERK, phospho-p38 (pp38), p38, and α-Tubulin. EphA2, ephrin type-A receptor 2.