Figure 3.

FAP promotes tumor invasion and liver metastasis

(A) Wound-healing assay showed that cell motility was enhanced following FAP overexpression in LS174T cells. Representative images from wounds made using a confluent monolayer of LS174T overexpressing FAP and control cells taken at 0 h and 24 h post wounding. The length of scale bar is 500 μm.

(B) The Boyden chamber assay was performed to investigate the effect of FAP on LS174T cells motility. The length of scale bar is 100 μm.

(C) FAP enhanced liver metastasis of LS174T cells. The tumor burden on liver was quantified by in vivo bioluminescence imaging using an IVIS Spectrum-CT.

(D) Tumor burden quantified weekly for LS174T overexpressing FAP, controls by in vivo bioluminescent imaging. Data are expressed as mean ± SD (n = 6). A mixed-effect linear model was used to determine significance of differences in tumor growth, p <0.05.

(E) In vivo 3D bioluminescence imaging. See also Figure S2.