Figure 2.

PDLC integrin and focal adhesion expression in AO (gray bars) and MO hydrogels (white bars). A) Representative western blots of protein from AO and MO hydrogels at 3 and 7 days. B-E) Quantification of western blot signals at 3 (B, D) and 7 days (C, E) using $\text{β}$-Actin as loading control. N=6 replicates, n=18 hydrogels. F) Representative images of PDLCs in optimized hydrogels at 3 and 7 days with actin cytoskeleton stained red (Phalloidin), nuclei blue (DAPI), and active integrin $\text{β1}$ green. Scale bars are 20 μm. G-H) Total fluorescent integrin $\text{β1}$ signal given as integrated density for individual PDLCs at 3 (G) and 7 days (H). I-J) number of discrete integrin $\text{β1}$ stained clusters in individual PDLCs at 3 (I) and 7 days (J). N=4 hydrogels, n=32 PDLCs. Unpaired t test. *p<0.05, ***p<0.001, ****p<0.0001.