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. Author manuscript; available in PMC: 2023 May 19.
Published in final edited form as: Biomater Adv. 2022 Aug 31;141:213093. doi: 10.1016/j.bioadv.2022.213093

Figure 4.

Figure 4.

Pyrophosphate regulation in AO (gray bars) and MO (white bars) hydrogels. A) ALP activity, B) Relative ENPP1 activity and C) Extracellular pyrophosphate concentrations at 1, 2, and 3 weeks culture. N=6 hydrogels. D-F) PDLC gene expression relative to expression 1 day after encapsulation using RPL32 as housekeeping gene. N=6 replicates, n=12 hydrogels. Two-way ANOVA with Tukey post hoc. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 for between group comparisons, # p<0.05, & p<0.01, $ p<0.001, % p<0.0001 for within group comparisons.