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. Author manuscript; available in PMC: 2023 May 19.
Published in final edited form as: Biomater Adv. 2022 Aug 31;141:213093. doi: 10.1016/j.bioadv.2022.213093

Figure 5.

Figure 5.

PDLC response to β-glycerophosphate in AO (gray bars) and MO (white bars) hydrogels at 1 (A-C) and 3 weeks (D-F). A,D) ALP activity at 1 (A) and 3 weeks (D), B,E) Relative ENPP1 activity at 1 (B) and 3 weeks (E). C,F) Pyrophosphate concentrations at 1 (C) and 3 weeks (F). G) Mineralization at 3 weeks. H) Representative z-stack images of calcium deposits (Xylenol orange) in hydrogels at 3 weeks. PDLC nuclei are stained blue with Hoechst 33342. Scale bars are 100 μm. N=3–6 hydrogels. Two-way ANOVA with Tukey post hoc. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 for between group comparisons, & p<0.01, $ p<0.001, % p<0.0001 for within group comparisons.