Figure 5.

Mitochondrial alterations in Tn and Tfh TC cells

Mitochondrial mass (A), mROS (B), and (MMP (C), in Tn and Tfh cells from B6 and TC mice. Each symbol represents a mouse (n = 8–15 pooled from 2 to 4 cohorts).

(D–F) Number of mitochondria per cell (D) and mitochondria size (E) in Tn and Teff assessed by electron microscopy. Representative images are shown in (F) at x15,000–20,000 (top) and ×40,000 (bottom) magnification. Black and red arrow heads point to individual B6 and TC mitochondria, respectively. In (D), each point represents a cell (n = 7–10) from 5 mice per group, and (E) represents individual mitochondria from (F).

Means ± SEM analyzed by 1-way ANOVA with’ Dunnett’s T3 multiple comparisons tests. ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.