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Plasmids used in this study and their relevant properties
| Plasmid(s) in donor strain HB101 | Properties | Antibiotic resistance carried | Transfera | Junctions detected |
|---|---|---|---|---|
| RP4 | Wild-type | Kanamycin | + | + |
| pML123, pVWDG23110Δ0.2 | Tra1 + Tra2 | Chloramphenicol, kanamycin | + | + |
| pML123, pWP471 | Tra2 + protease TraF (pili processed) | Chloramphenicol, ampicillin | − | ± |
| pML123 | Tra2 only (pili unprocessed) | Chloramphenicol | − | ± |
| pML123mtrbC45 | Pilin− mutant (MURFI linker in trbC) | Chloramphenicol | − | + |
| None | — | None | − | − |
