KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-Ki67 | Abcam | Cat #Ab16667; RRID:AB_302459 |
| Mouse anti-p21 | BD biosciences | Cat #556431; RRID:AB_396415 |
| Rat anti-p19/Arf | Abcam | Cat #Ab174939; RRID:AB_2924683 |
| Mouse anti-p16 | Abcam | Cat #Ab54210; RRID:AB_881819 |
| Goat anti-IL-1 α | R&D systems | Cat #AF-400; RRID:AB_354473 |
| Rabbit anti-IL-1 β | Santa Cruz | Cat #sc-7884; RRID:AB_2124476 |
| Rabbit anti-SAA1 | Biorbyt | Cat #orb228668; RRID:AB_2924684 |
| Mouse anti-Nras | Santa Cruz | Cat #sc-31; RRID:AB_628041 |
| Rat anti-F4/80 | Abcam | Cat #Ab6640; RRID:AB_1140040 |
| Rabbit anti-CD3 | Abcam | Cat #Ab5690; RRID:AB_305055 |
| Rabbit anti-CD4 | Abcam | Cat #Ab183685; RRID:AB_2686917 |
| Rabbit anti-CD8 | Abcam | Cat #Ab217344; RRID:AB_2890649 |
| Rabbit anti-CD68 | Abcam | Cat #Ab125212; RRID:AB_10975465 |
| Rabbit anti-TLR2 | Novus Bio | Cat #NBP2-24861; RRID:AB_2924685 |
| Mouse anti-Human IL1B | Santa Crus | Cat #sc-32294; RRID:AB_627790 |
| Mouse anti-NF-κB p65 | Proteintech | Cat #66535-1-Ig; RRID:AB_2881898 |
| Biotinylated goat anti-mouse IgG | Vector labs | Cat #BA9200; RRID:AB_2336171 |
| Biotinylated goat anti-rabbit IgG | Vector labs | Cat #BA1000; RRID:AB_2313606 |
| Biotinylated rabbit anti-goat IgG | Vector labs | Cat #BA5000; RRID:AB_2336126 |
| Anti-rabbit HRP polymer | Dako | Cat #K4003; RRID:AB_2630375 |
| Goat anti-mouse AF488 | ThermoFisher | Cat #A11029; RRID:AB_2534088 |
| Goat anti-rat AF594 | ThermoFisher | Cat #A11007; RRID:AB_10561522 |
| Mouse monoclonal anti-α-tubulin | Sigma | Cat #T9026; RRID:AB_477593 |
| Anti-rabbit IgG HRP-linked | CST | Cat #7074S; RRID:AB_2099233 |
| Anti-mouse IgG HRP-linked | CST | Cat #7076S; RRID:AB_330924 |
| Anti-mouse CD45 (Brilliant Violet 421) | Biolegend | Cat #103133; RRID:AB_10899570 |
| Anti-mouse CD3 (PE/Cyanine7) | Biolegend | Cat #100219; RRID:AB_1732068 |
| Anti-mouse CD4 (PE) | Biolegend | Cat #100407; RRID:AB_312692 |
| Anti-mouse CD8 (Super bright 600) | Invitrogen | Cat #63-0081-80; RRID:AB_2637162 |
| Anti-mouse TCR γδ (Alexa Fluor® 488) | Biolegend | Cat #118127; RRID:AB_2562770 |
| Anti-mouse/human CD11b (APC) | Biolegend | Cat #101211; RRID:AB_312794 |
| Anti-mouse CD11c (APC/Cyanine7) | Biolegend | Cat #117323; RRID:AB_830646 |
| Anti-mouse Siglec-F (PE) | Biolegend | Cat #155505; RRID:AB_2750234 |
| Anti-mouse Ly6C (FITC) | Biolegend | Cat #128005; RRID:AB_1186134 |
| Anti-mouse Ly6G (Alexa Fluor® 700) | Biolegend | Cat #127621; RRID:AB_10640452 |
| Anti-mouse CD19 (PE/Cyanine7) | Biolegend | Cat #115519; RRID:AB_313654 |
| Anti-mouse CD335 (PE/Cyanine7) | Biolegend | Cat #137617; RRID:AB_11218594 |
| 7AAD viability staining solution | Biolegend | Cat #420403 |
| Bacterial and virus strains | ||
| Adeno-CMV-CRE | Iowa Viral Vector Core | VVC-U of Iowa-5 |
| Biological samples | ||
| Human lung adenocarcinoma samples | NRS Lothian Bioresource | Bioresource ref: SR1382 |
| Human lung squamous carcinoma-in-situ samples | UCLH early lung cancer surveillance program | REC ref: 06/Q0505/12 and 01/0148 |
| Chemicals, peptides, and recombinant proteins | ||
| Anti-Digoxigenin-AP | Roche | Cat #11093274910 |
| Proteinase K | Roche | Cat #03115879001 |
| NBT | Promega | Cat #S3771 |
| BCIP | Roche | Cat #16853423 |
| Experimental models: Organisms/strains | ||
| B6.129-Tlr2tm1Kir/J | Jax.org | 004650 |
| B6.129S4-Krastm4Tyj/J | Jax.org | 008179 |
| B6.129- Krastm4Tyj Trp53tm1Brn/J | Jax.org | 032435 |
| Oligonucleotides | ||
| Primers for qPCR; see Table S1 | This paper | N/A |
| gRNA sequence for pSECC; see Table S2 | This paper | N/A |
| Primers for mouse Tlr2 RNA-ISH; see Table S3 |
This paper | N/A |
| Software and algorithms | ||
| QuPath | Bankhead et al., 2017 | https://qupath.github.io |