Figure 2.
Results from SEC on MBP-Vpu protein in buffered aqueous solution. The SEC results demonstrate that all the MBP-FL Vpu, MBP-VpuΔh3 and MBP-VpuΔh2h3 constructs form oligomers, as they elute predominantly at about 8.5 – 12 ml (depending on the construct) and only small fraction elutes as monomers corresponding to the peak at 16 – 18 ml. The protein concentration was 15 μM for all the three constructs.