Figure 1.
A schematic illustration of transcriptional and translational reporters of alx and mntP. A transcriptional reporter contains a promoter (P) of aix or mntP, followed by the 5’ UTR (PRE In the case of alx), a ribosome binding site (RBS) of the gene of Interest Indicated by a filled circle in green, the first X nucleotides of the gene of interest (53 and 47 nt for alx and mntP, respectively), and a stop codon indicated by a rectangular box in magenta, followed by lacZ gene with its own RBS indicated by a rectangular box and filled circle respectively in blue. A translational reporter contains a promoter (P) of alx or mntP is followed by a 5’ UTR element (PRE in the case of alx), an RBS of the gene of interest indicated by a filled circle in blue, and first Y nucleotides of the gene of interest (99 and 47 nt for alx and mntP, respectively) fused to in frame with the 8th codon of lacZ gene.