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[Preprint]. 2023 May 8:2023.05.07.539761. [Version 1] doi: 10.1101/2023.05.07.539761

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Figure 3. — Effect of increased external pH on growth, cytoplasmic pH, and cellular oxidative stress. (A) Growth of the parent strain (MC4100) and Its derivatives (Δalx::Kan derivative, RAS31; AmntP::Kan derivative, RAS32, and Δalx AmntP::Kan derivative, RAS42) in LBK media with pH 6.8 and pH 8.4. (B) Measurements of cytoplasmic pH in the parent strain (MC4100) and its Δalx::Kan derivative (RAS31) expressing pHluorin in M63A media with varying pH. β-galactosidase activity (Miller units) as a reporter of katG transcription was measured for mid-log phase grown cultures of the parent strain (AL441) and its Δalx::Kan derivative (RAS136) cultivated In LBK media with pH 6.8 and 8.4 (panel C) or pH 6.8 LB with and without supplemented MnCh (panel D). The error shown is standard deviation of three repeats of the experiment.