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[Preprint]. 2023 May 9:2023.05.08.539917. [Version 1] doi: 10.1101/2023.05.08.539917

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Figure 2. — (A) The F350/F330 thermal unfolding curve for DsbB reconstituted in a dark nanodisc model membrane system (violet). The thermal unfolding curve for empty dark nanodiscs is shown in grey. (B) The first derivative plot of the F350/F330 ratio with respect to temperature for DsbB in a nanodisc. The inflection points correspond to T_m values of 70.5 °C and 77.5 °C, respectively. All samples were run in triplicate for three independent preparations and the average is plotted. (C) Representative SDS-PAGE gel following size exclusion to showcase the successful reconstitution of DsbB in a dark nanodisc.