Skip to main content
[Preprint]. 2023 May 9:2023.05.08.539917. [Version 1] doi: 10.1101/2023.05.08.539917

Figure 4. NanoDSF measurements for DsbB reconstituted in dark nanodiscs under reducing conditions.

Figure 4.

(A) The F350/F330 thermal unfolding curve of DsbB reconstituted in the dark nanodisc in the presence of the reducing agent TCEP at 2 mM. (B) The first derivative plot shows a single inflection point corresponding to a Tm value of 76.6 ± 0.5 °C. The first derivative plot for DsbB reconstituted in the dark nanodisc in the absence of reducing conditions has been overlayed as a comparison. All samples were run in triplicate and the average is plotted.