Skip to main content
[Preprint]. 2023 May 11:2023.05.08.23289640. [Version 1] doi: 10.1101/2023.05.08.23289640

Figure 7. ABLIM1 is a common ILD autoantigen with high expression in fibrotic lungs.

Figure 7.

a. Representative immunofluorescence staining of explanted IPF lungs for Hoechst, alpha-smooth muscle actin (α-SMA), cytokeratin 17 (KRT-17), and ABLIM1. Images were taken with an 40x objective, and the scale bar is 100um. b. Close-up view of a region of IPF lung where there is the co-localization of ABLIM1+ cells and KRT-17+ de-differentiated airway basal cells. The scale bar is 100um. c. Quantification of plasma/serum antibody binding to the full-length, radiolabeled ABLIM1 protein. The horizontal line is placed at three standard deviations above the mean value for healthy controls (HC), which was used as a cutoff to determine positivity. Samples from rheumatoid arthritis (RA) patients with no symptoms of lung disease and type 1 diabetes patients (T1D) were included as comparison groups, and healthy controls (HC) are samples donated through NY Blood Bank. d-e. Linear regression between anti-ABLIM1 index in serum and Tfh cell (d) and Treg cell (e) proportions in ILD LLNs.