Fig. 7.

Thailandamide is bioactive against F. johnsoniae . While the nonsynonymous mutation in tolC did not reduce antibiotic susceptibility (present in F. johnsoniae coevolved line 3), F. johnsoniae coevolved line 3 still displayed a reduction in antibiotic susceptibility at the end of plate passage five (A). B. thailandensis transposon mutants in thaF (B) and thaA (C) have decreased inhibition toward F. johnsoniae while an atsR transposon mutant has increased inhibition of F. johnsoniae (D). Below the panels is an amino acid sequence alignment between the F . johnsoniae AccA and S. enterica AccA. The asterisks indicate positions within the proteins that were identical. The blue box highlights the alignment of P160 in F. johnsoniae and P164 in S. enterica .