Table 3.
Strains and plasmids used in this study
|
Strain or plasmid |
Description |
Source or reference |
|---|---|---|
|
Escherichia coli strains |
||
|
DH5αMCR |
Strain used for general cloning |
Life Technologies (Grand Island, NY, USA) |
|
HB101 |
Strain used with pRK2013 for triparental conjugation |
[63, 64] |
|
DH5αMCR_pYT354 |
pYT354 in DH5amcr; sacB-containing suicide vector |
[29] |
|
B. thailandensis strain |
||
|
E264 (ATCC 700388) |
Wild-type |
[65] |
|
F. johnsoniae strains |
||
|
UW101 (ATCC 17061) |
Wild-type |
[66] |
|
CoE_Δ33_tolC |
Coevolved strain containing a 33 bp (261–293) deletion in tolC (FJOH_RS06580) |
This study |
|
CoE_G247A_tolC |
Coevolved strain containing a nonsynonymous mutation (G247A) in tolC (FJOH_RS06580) |
This study |
|
CoE_792_793insG_ragB |
Coevolved strain containing an insertion (G) between bp 792 and 793 in ragB/susD (FJOH_RS24865) |
This study |
|
reΔ33_tolC |
tolC (FJOH_RS06580) 33 bp deletion placed in ancestor |
This study |
|
reG247A_tolC |
tolC (FJOH_RS06580) G247A mutation placed in ancestor |
This study |
|
re792_793insG_ragB |
ragB/susD (FJOH_RS24865) bp (G) insertion placed between bp 792 and 793 in ancestor |
This study |
|
Plasmids |
||
|
pYT354 |
sacB-containing suicide vector; Apr (Emr). pYT354 is modified from pYT313 with different multiple cloning sites |
[29] |
|
pJC101 |
Construct used to replace ancestral native tolC (FJOH_RS06580) with tolC 33 bp deletion |
This study |
|
pJC102 |
Construct used to replace ancestral native tolC (FJOH_RS06580) with tolC G247A |
This study |
|
pJC103 |
Construct used to replace ancestral native ragB/susD (FJOH_RS24865) with ragB/susD 792_793insG |
This study |
|
pRK2013 |
Helper plasmid for triparental conjugation; Kmr |
[64] |
Apr, ampicillin resistance, 100 μg ml−1; Kmr, kanamycin resistance, 30 μg ml−1; Emr, erythromycin resistance, 100 μg ml−1 for F. johnsoniae. Antibiotics resistance phenotypes listed in parentheses (Em) are those expressed in F. johnsoniae but not in E. coli. Antibiotics resistance phenotypes listed not in parentheses (Ap, Km) are those expressed in E. coli but not in F. johnsoniae.