FIG. 1.

Western blots showing cellular levels of DivIC in BB11 (Pspac-ftsZ) (A) and in the divIB null mutant, SU321, and its wild-type parent strain, SU5 (B). (A) BB11 was grown at 37°C in IPTG-containing medium (0 min) and resuspended in either IPTG-lacking or IPTG-containing medium for 20, 40, 60, and 90 min. (B) SU321 and SU5 were shifted from 30°C at mid-exponential phase to 49°C for 1 h. Samples were collected prior to (0 min) and 20, 40, and 60 min after the shift. A₅₉₀ equivalents were loaded in each case. Standard protein positions are expressed in kilodaltons.