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DivIC and FtsZ localization in SU321 (divIB null) grown at 30°C to mid-exponential phase and then shifted to 49°C for 1 h. (B and D) Cells immunostained with fluorescein isothiocyanate; (A and C) phase-contrast image of the same field. DivIC localization is shown in panel B, while FtsZ localization is shown in panel D. Cells grown at 30°C (short) were mixed with long cells (indicated by arrowheads) that had been shifted to 49°C for 1 h. Bar = 5 μm (A).
