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. Author manuscript; available in PMC: 2023 May 19.
Published in final edited form as: Nat Biomed Eng. 2021 Oct 6;5(9):1059–1068. doi: 10.1038/s41551-021-00786-x

Fig. 2 ∣. Surveying how four LNP chemical traits influence nebulized lung mRNA delivery in vivo.

Fig. 2 ∣

a–e, We varied the structure and amount of PEG-lipid, the phospholipid charge and cholesterol within the LNP (a), while the 7C1 compound was kept constant (b). In this way, we created and analyzed six extreme groups (c) using LNP quality control (QC) cutoffs (d), of which three consistently led to stable LNPs as measured by the hydrodynamic diameter of all LNPs for all six groups. e, In groups D–F, we found that the diameter of the pooled LNPs was within the range of the individual LNPs making up the groups, suggesting their stability. The dotted grey line represents a 200 nm QC cutoff. f,g, Luciferase (Luc) luminescence images (f) and quantification (g) in lungs 48 h after the three groups were nebulized and administered to mice, as compared to a negative control (−Ctrl). Unless specified otherwise, *P = 0.0098, one-way analysis of variance (ANOVA), average ± s.e.m., n = 2 mice per group, biological replicates shown.