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. 2023 May 5;14(7):1223–1231. doi: 10.7150/jca.83615

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CD4⁺T and CD8⁺T cells in malignant effusion expressed lower levels of Granzyme B and Perforin than that in blood. The mononuclear cells from malignant effusion and blood were prepared and stained with anti-CD45, CD3, CD4, CD8, Granzyme B and Perforin, and assessed by FACS. Gated on CD3⁺CD4⁺T (A, C) and CD3⁺CD8⁺T cells (B, D), the representative graph and statistical results for the expression Granzyme B and Perforin were shown. Statistical results were shown as mean±SEM. The statistical significance was determined with the Mann-Whitney U test. NS, no significance; *P < 0.05; ***P < 0.001.