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. Author manuscript; available in PMC: 2023 May 19.
Published in final edited form as: Cell Rep. 2023 Feb 26;42(3):112175. doi: 10.1016/j.celrep.2023.112175

Figure 7. Human CD8+ T cells exhibit differential adhesion to P-selectin in physiological levels of fluid flow during expansion.

Figure 7.

(A) Expansion of human CD8+ T cells from healthy donors cultured with Dynabeads and IL-2.

(B) Percentage of CD8+ T cells recovered in Adh and FF fractions using P-selectin-functionalized channel.

(C) Frequency of P-selectin ligand+ cells of live CD8+ T cells of parent populations or those recovered in FF or Adh fractions after perfusion through a P-selectin-functionalized channel at different days of expansion.

(D) Histograms of P-selectin ligand expression at different days of expansion of human CD8+ T cells.

(E) Mean fluorescence intensity of P-selectin ligand+ cells normalized to parent cell mean fluorescence. For all graphs the data represent the mean ± SEM of three or more independently run experiments; two-way ANOVA with Bonferroni’s multiple comparisons test; *p < 0.05, **p < 0.01, ****p < 0.0001. See also Figure S16.