KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| Goat anti-human IgG (Fc Specific) | Sigma-Aldrich | Cat#I2136 |
| Goat anti-mouse CD62P | R&D Systems | Cat#AF737 |
| Donkey anti-goat Alexa Fluor 555 | Invitrogen | Cat#A21432; RRID:AB_2535853 |
| Rat anti-mouse CD3 | Invitrogen | Cat#14–0032-82; RRID:AB_467053 |
| Rat anti-mouse CD31 | Invitrogen | Cat#14–0311-82; RRID:AB_467201 |
| Donkey anti-rat Alexa Flour 647 | Invitrogen | Cat#A78947; RRID:AB_2910635 |
| InVivoPlus anti-mouse PD-1 (CD279) | BioXCell | Cat#BP0146; RRID:AB_10949053 |
| FITC anti-IgG (Fc specific) | Sigma-Aldrich | Cat#F9512 |
| APC anti-mouse CD44 | Biolegend | Cat#103012; RRID:AB_312963 |
| BV421 anti-mouse CD62L | Biolegend | Cat#104436; RRID:AB_2562560 |
| APC/Cyanine7 anti-mouse CD8a | Biolegend | Cat#100714; RRID:AB_312753 |
| BV785 anti-mouse CCR7 | Biolegend | Cat#145526; RRID:AB_2566799 |
| PE/Cyanine anti-mouse LFA-1 | Biolegend | Cat#141012; RRID:AB_2564308 |
| PE anti-mouse PSGL-1 | BD Biosciences | Cat#555306; RRID:AB_395719 |
| PerCP anti-mouse CD3 | Biolegend | Cat#100326; RRID:AB_893317 |
| PE anti-mouse CD45.1 | Biolegend | Cat#110708; RRID:AB_313497 |
| BV711 anti-mouse CD45.2 | Biolegend | Cat#109847; RRID:AB_2616859 |
| AF700 anti-mouse SCA-1 | Biolegend | Cat#108142; RRID:AB_2565959 |
| BV605 anti-mouse PD-1 | Biolegend | Cat#135220; RRID:AB_2562616 |
| AF700 anti-mouse Ki-67 | Biolegend | Cat#652420; RRID:AB_2564285 |
| FITC anti-mouse Granzyme B | Biolegend | Cat#372206; RRID:AB_2687030 |
| PE anti-mouse IFN-ɣ | Biolegend | Cat#505808; RRID:AB_315402 |
| PE-Cy7 anti-mouse TNF-α | Biolegend | Cat#506324; RRID: AB_2256076 |
| APC-Cy7 anti-Human CD8 | BD Biosciences | Cat#557834; RRID:AB_396892 |
| APC anti-human CD62L | Biolegend | Cat#304809; RRID:AB_314469 |
| PE anti-human HECA-452 (sLea/x) | Biolegend | Cat#321312; RRID:AB_2565589 |
| BV605 anti-human CCR7 | Biolegend | Cat#353223; RRID:AB_11124325 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Recombinant Mouse P-selectin Fc Chimera | R&D Systems | Cat#737-PS-200 |
| Recombinant Mouse ICAM Fc Chimera | R&D Systems | Cat#796-IC-050 |
| Recombinant Human P-selectin Fc Chimera | R&D Systems | Cat#137-PS-050 |
| Polydimethylsiloxane (PDMS), Dow Corning Sylgard 184 | Ellsworth Adhesives | Cat#184 SIL ELAST KIT 3.9KG, SKU 2065622 |
| Phorbol 12-myristate 13-acetate | Sigma-Aldrich | Cat#P8139–1MG |
| Ionomycin, Calcium Salt | Invitrogen | Cat#I24222 |
| Recombinant-IL-2 | Bio-techne | Cat#402-ML-020 |
| SIINFEKL | vivitide | Cat#257–264 |
|
| ||
| Critical commercial assays | ||
|
| ||
| MojoSort Mouse CD8a Selection Kit | Biolegend | Cat#480035 |
| EasySep Human CD8+ T cell Enrichment Kit | STEMCELL | Cat#19053 |
| Dynabeads Mouse T-Activator CD3/CD28 | Gibco | Cat#11453D |
| Dynabeads Human T-Activator CD3/CD28 | Gibco | Cat#11132D |
| EasySep Dead Cell Removal (Annexin V) Kit | STEMCELL | Cat#17899 |
| eBioscience Foxp3/Transcription Factor Staining Buffer Set | ThermoFisher | Cat#00–5523-00 |
| ZombieAqua Fixable Viability Kit | Biolegend | Cat#423102 |
|
| ||
| Experimental models: Cell lines | ||
|
| ||
| B16F10 | Obtained from Swartz Laboratory | N/A |
| B16F10-OVA | Obtained from Swartz Laboratory | N/A |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| 6 week old female C57BL/6J | Jackson Labs | Strain#000664 |
| 6 week old female B6 CD45.1 | Jackson Labs | Strain#002014 |
| OT-I Mouse | Charles River | Strain Code 642 |
|
| ||
| Software and algorithms | ||
|
| ||
| FlowJo | Treestar, Inc | N/A |
| ImageJ | National Institutes of Health | N/A |
| Manual Tracking ImageJ plugin | National Institutes of Health | N/A |
| Prism 9 | GraphPad | N/A |
|
| ||
| Other | ||
|
| ||
| Culture Dishes, Nontreated Sterile | Corning Life Sciences | Cat#431272 |
| Double sided adhesive 96042 | 3M | 3M ID 700063936593 |
| Non-silicone secondary release | 3M | 3M ID 70000118912 |