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. Author manuscript; available in PMC: 2023 May 19.
Published in final edited form as: Biochim Biophys Acta. 2009 May 3;1793(7):1272–1278. doi: 10.1016/j.bbamcr.2009.04.013

Fig. 1.

Fig. 1.

Specificity of anti-peptide antibodies. A. Protein blots of in vitro translated products of LOX expression clones and bacterial expression product probed with affinity purified antibodies. C, no DNA control translation; 30, product from mature LOX clone; 50, product from proenzyme clone; 18, in vitro translation of propeptide clone; 18b, propeptide clone bacterial product. Anti-LOpep4 binds non-specifically to a wheat germ protein slightly smaller than 50-kDa, and anti-LOX41568 binds non-specifically to a wheat germ product of about 40-kDa. B. Enzymatically active bovine heart LOX protein probed with antibodies to mature LOX epitopes. LO, 25 ng bovine LOX preparation; M, protein size markers.