RNA interference reduces LOX message and proenzyme protein. A. RNA blot probed with LOX DNA probe. GAPDH used as loading control. 1. NIH 3T3; 2. RS485; 3–6. shRNA clones 2–2A-2, 3–1A-4, 3–1B-14, and 3–1B-17. B. Total protein extracts probed with the indicated antibody. Actin used as loading control. M, protein size markers. 1. NIH 3T3; 2. RS485; 3–6. shRNA clones 2–2A-2, 3–1A-4, 3–1B-14, and 3–1B-17.